![]() The main factors explaining those differences are the size of the pond, its tree canopy and the seasonality of the water table, which strongly determines the Ericaceae and Pinus percentages. The average pollen percentages of all sediment samples studied allows a fairly good reconstruction of the main vegetation units in the area, but major differences between the average samples obtained in each sedimentary system, and also between some samples taken from the same system are found. To determine whether modern pollen content in sediments from upland lake systems reflects the factual regional and extra-local vegetation composition, we analysed twenty-five samples focusing on aspects such as pond size and morphology, relative position in the catchment, local vegetation, canopy configuration and seasonality of the water table. Wetter conditions were inferred to exist during the Greenland interstadials, while drier conditions persisted during Heinrich events. A comparison with long records from the Greenland ice cores, the marine Cariaco Basin and insolation parameters revealed that the pollen data and the Pinus pollen accumulation rates evidence the response of the vegetation to glacial and interglacial changes and to millennial-scale variability. ![]() Tree-line changes in response to climate change and taxa migration were documented. During MIS 4 and 3, a trend towards drier conditions was also recorded with enhanced carbonate precipitation and proxies indicating saline conditions. MIS 5c to MIS 3 showed lower and variable evenness and palynological richness, along with an increase in fire activity. Pinus-dominated forests characterize the landscape during MIS 5a. Open communities and Pinus and Quercus forests with mesophytic taxa and, during the stadial periods, MIS 5 d, 5c, and 5 b (125–90 ka), with tropical taxa, characterize the record of interstadials. The penultimate interglacial MIS 5e (126.5–123.6 ka) shows a dominance of Pinus forests, with tropical taxa suggesting warmer than present conditions environmental proxies indicate drier conditions and that the lake transitioned into a saline water body. ![]() The wettest cold stage, indicated by a combination of diverse open vegetation and mesophytic taxa correlates with the penultimate late glacial MIS 6 (146–130 ka), when Lake Chalco was a deep freshwater lake. The changes in plant composition were reconstructed by pollen analysis and differences in plant composition between distinct periods were established. The pollen record displays a high temporal resolution with a mean sampling interval of ∼230 years. This record provides an insight into vegetation dynamics and climate variability at the northern limit of the American tropics. A new detailed pollen record of the penultimate glacial–interglacial–glacial cycle corresponding to the late marine isotopic stages (MISs) 6–3 (146–35 ka ago) has been obtained from Lake Chalco, in the Basin of Mexico.
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