Many techniques that evolve to be standardized tools in the biological sciences go through a period of transformation in which they become, at least to some degree, automated, in order to improve reproducibility, throughput and standardization. ![]() The patch clamp technique has been an invaluable tool since its inception several decades ago and has generated a wealth of knowledge about the nature of voltage- and ligand-gated ion channels, sub-threshold and supra-threshold activity, and characteristics of action potentials related to higher order functions. Despite great progress in imaging technologies and sensor proteins, none of the currently available tools allow imaging of neural activity on a millisecond timescale and beyond the first few hundreds of microns inside the brain. Novel deep learning and image analysis algorithms for automated patch clamp systems to reliably measure neurons in human and rodent brain slices.Įlectrophysiology is the study of neural activity in the form of local field potentials, current flow through ion channels, calcium spikes, back propagating action potentials and somatic action potentials, all measurable on a millisecond timescale. Our tool can multiply the number of daily measurements to help brain research. The software has a diary module that automatically logs patch clamp events. We also demonstrate that further molecular and anatomical analysis can be performed on the recorded cells. High-quality measurements were performed on hundreds of human and rodent neurons. The pipette tip detection and approaching phase use image analysis techniques for precise movements. The model was trained on a new image database of neurons in unlabeled brain tissue slices. ![]() The cell detection is based on deep learning. The automation covers the detection of cells in label-free images, calibration of the micropipette movement, approach to the cell with the pipette, formation of the whole-cell configuration, and recording. We have developed a tool that fully automatically performs electrophysiological recordings in label-free tissue slices. Patch clamp recording of neurons is a labor-intensive and time-consuming procedure.
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